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Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues.

Identifieur interne : 001464 ( Main/Exploration ); précédent : 001463; suivant : 001465

Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues.

Auteurs : Ajaya K. Biswal [États-Unis] ; Li Tan [États-Unis] ; Melani A. Atmodjo [États-Unis] ; Jaclyn Demartini [États-Unis] ; Ivana Gelineo-Albersheim [États-Unis] ; Kimberly Hunt [États-Unis] ; Ian M. Black [États-Unis] ; Sushree S. Mohanty [États-Unis] ; David Ryno [États-Unis] ; Charles E. Wyman [États-Unis] ; Debra Mohnen [États-Unis]

Source :

RBID : pubmed:28725262

Abstract

BACKGROUND

The effective use of plant biomass for biofuel and bioproduct production requires a comprehensive glycosyl residue composition analysis to understand the different cell wall polysaccharides present in the different biomass sources. Here we compared four methods side-by-side for their ability to measure the neutral and acidic sugar composition of cell walls from herbaceous, grass, and woody model plants and bioenergy feedstocks.

RESULTS

Arabidopsis,

CONCLUSIONS

This work provides a side-by-side comparison of the efficacy of four different established glycosyl residue composition analysis methods in the analysis of the glycosyl residue composition of cell walls from both dicot (Arabidopsis and


DOI: 10.1186/s13068-017-0866-1
PubMed: 28725262
PubMed Central: PMC5513058


Affiliations:


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Le document en format XML

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<name sortKey="Black, Ian M" sort="Black, Ian M" uniqKey="Black I" first="Ian M" last="Black">Ian M. Black</name>
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<author>
<name sortKey="Mohanty, Sushree S" sort="Mohanty, Sushree S" uniqKey="Mohanty S" first="Sushree S" last="Mohanty">Sushree S. Mohanty</name>
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<name sortKey="Ryno, David" sort="Ryno, David" uniqKey="Ryno D" first="David" last="Ryno">David Ryno</name>
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<region type="state">Tennessee</region>
</placeName>
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</affiliation>
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<author>
<name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name>
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<nlm:affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</nlm:affiliation>
<country>États-Unis</country>
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<region type="state">Tennessee</region>
</placeName>
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<wicri:noRegion>Oak Ridge</wicri:noRegion>
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<nlm:affiliation>Center for Environmental Research and Technology (CE-CERT) and Department of Chemical and Environmental Engineering, University of California Riverside, Riverside, 92507 CA USA.</nlm:affiliation>
<country>États-Unis</country>
<placeName>
<region type="state">Californie</region>
</placeName>
<wicri:regionArea>Center for Environmental Research and Technology (CE-CERT) and Department of Chemical and Environmental Engineering, University of California Riverside, Riverside</wicri:regionArea>
<wicri:noRegion>Riverside</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Mohnen, Debra" sort="Mohnen, Debra" uniqKey="Mohnen D" first="Debra" last="Mohnen">Debra Mohnen</name>
<affiliation wicri:level="2">
<nlm:affiliation>Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName>
<region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Department of Biochemistry and Molecular Biology, University of Georgia, Athens</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2">
<nlm:affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</nlm:affiliation>
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<region type="state">Géorgie (États-Unis)</region>
</placeName>
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<country>États-Unis</country>
<placeName>
<region type="state">Tennessee</region>
</placeName>
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<wicri:noRegion>Oak Ridge</wicri:noRegion>
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<series>
<title level="j">Biotechnology for biofuels</title>
<idno type="ISSN">1754-6834</idno>
<imprint>
<date when="2017" type="published">2017</date>
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<front>
<div type="abstract" xml:lang="en">
<p>
<b>BACKGROUND</b>
</p>
<p>The effective use of plant biomass for biofuel and bioproduct production requires a comprehensive glycosyl residue composition analysis to understand the different cell wall polysaccharides present in the different biomass sources. Here we compared four methods side-by-side for their ability to measure the neutral and acidic sugar composition of cell walls from herbaceous, grass, and woody model plants and bioenergy feedstocks.</p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>RESULTS</b>
</p>
<p>Arabidopsis, </p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>CONCLUSIONS</b>
</p>
<p>This work provides a side-by-side comparison of the efficacy of four different established glycosyl residue composition analysis methods in the analysis of the glycosyl residue composition of cell walls from both dicot (Arabidopsis and </p>
</div>
</front>
</TEI>
<pubmed>
<MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM">
<PMID Version="1">28725262</PMID>
<DateRevised>
<Year>2020</Year>
<Month>10</Month>
<Day>01</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection">
<Journal>
<ISSN IssnType="Print">1754-6834</ISSN>
<JournalIssue CitedMedium="Print">
<Volume>10</Volume>
<PubDate>
<Year>2017</Year>
</PubDate>
</JournalIssue>
<Title>Biotechnology for biofuels</Title>
<ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation>
</Journal>
<ArticleTitle>Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues.</ArticleTitle>
<Pagination>
<MedlinePgn>182</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-017-0866-1</ELocationID>
<Abstract>
<AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The effective use of plant biomass for biofuel and bioproduct production requires a comprehensive glycosyl residue composition analysis to understand the different cell wall polysaccharides present in the different biomass sources. Here we compared four methods side-by-side for their ability to measure the neutral and acidic sugar composition of cell walls from herbaceous, grass, and woody model plants and bioenergy feedstocks.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Arabidopsis,
<i>Populus</i>
, rice, and switchgrass leaf cell walls, as well as cell walls from
<i>Populus</i>
wood, rice stems, and switchgrass tillers, were analyzed by (1) gas chromatography-mass spectrometry (GC-MS) of alditol acetates combined with a total uronic acid assay; (2) carbodiimide reduction of uronic acids followed by GC-MS of alditol acetates; (3) GC-MS of trimethylsilyl (TMS) derivatives; and (4) high-pressure, anion-exchange chromatography (HPAEC). All four methods gave comparable abundance ranking of the seven neutral sugars, and three of the methods were able to quantify unique acidic sugars. The TMS, HPAEC, and carbodiimide methods provided comparable quantitative results for the specific neutral and acidic sugar content of the biomass, with the TMS method providing slightly greater yield of specific acidic sugars and high total sugar yields. The alditol acetate method, while providing comparable information on the major neutral sugars, did not provide the requisite quantitative information on the specific acidic sugars in plant biomass. Thus, the alditol acetate method is the least informative of the four methods.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This work provides a side-by-side comparison of the efficacy of four different established glycosyl residue composition analysis methods in the analysis of the glycosyl residue composition of cell walls from both dicot (Arabidopsis and
<i>Populus</i>
) and grass (rice and switchgrass) species. Both primary wall-enriched leaf tissues and secondary wall-enriched wood/stem tissues were analyzed for mol% and mass yield of the non-cellulosic sugars. The TMS, HPAEC, and carbodiimide methods were shown to provide comparable quantitative data on the nine neutral and acidic sugars present in all plant cell walls.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y">
<Author ValidYN="Y">
<LastName>Biswal</LastName>
<ForeName>Ajaya K</ForeName>
<Initials>AK</Initials>
<AffiliationInfo>
<Affiliation>Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Tan</LastName>
<ForeName>Li</ForeName>
<Initials>L</Initials>
<AffiliationInfo>
<Affiliation>Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Atmodjo</LastName>
<ForeName>Melani A</ForeName>
<Initials>MA</Initials>
<AffiliationInfo>
<Affiliation>Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
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<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y">
<LastName>DeMartini</LastName>
<ForeName>Jaclyn</ForeName>
<Initials>J</Initials>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>Center for Environmental Research and Technology (CE-CERT) and Department of Chemical and Environmental Engineering, University of California Riverside, Riverside, 92507 CA USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2222 1582</Identifier>
<Identifier Source="GRID">grid.266097.c</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>DuPont Industrial Biosciences, Palo Alto, CA 94304 USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Gelineo-Albersheim</LastName>
<ForeName>Ivana</ForeName>
<Initials>I</Initials>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Hunt</LastName>
<ForeName>Kimberly</ForeName>
<Initials>K</Initials>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>South Georgia State College, Douglas, GA 31533 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 0395 0730</Identifier>
<Identifier Source="GRID">grid.469257.b</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Black</LastName>
<ForeName>Ian M</ForeName>
<Initials>IM</Initials>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Mohanty</LastName>
<ForeName>Sushree S</ForeName>
<Initials>SS</Initials>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Ryno</LastName>
<ForeName>David</ForeName>
<Initials>D</Initials>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Wyman</LastName>
<ForeName>Charles E</ForeName>
<Initials>CE</Initials>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>Center for Environmental Research and Technology (CE-CERT) and Department of Chemical and Environmental Engineering, University of California Riverside, Riverside, 92507 CA USA.</Affiliation>
<Identifier Source="ISNI">0000 0001 2222 1582</Identifier>
<Identifier Source="GRID">grid.266097.c</Identifier>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Mohnen</LastName>
<ForeName>Debra</ForeName>
<Initials>D</Initials>
<AffiliationInfo>
<Affiliation>Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Rd., Athens, GA 30602-4712 USA.</Affiliation>
<Identifier Source="ISNI">0000 0004 1936 738X</Identifier>
<Identifier Source="GRID">grid.213876.9</Identifier>
</AffiliationInfo>
<AffiliationInfo>
<Affiliation>DOE-BioEnergy Science Center (BESC), Oak Ridge, 37831 TN USA.</Affiliation>
</AffiliationInfo>
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<Language>eng</Language>
<PublicationTypeList>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
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<Year>2017</Year>
<Month>07</Month>
<Day>14</Day>
</ArticleDate>
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<Country>England</Country>
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<ISSNLinking>1754-6834</ISSNLinking>
</MedlineJournalInfo>
<KeywordList Owner="NOTNLM">
<Keyword MajorTopicYN="N">Biofuel</Keyword>
<Keyword MajorTopicYN="N">Cell wall</Keyword>
<Keyword MajorTopicYN="N">Feedstock</Keyword>
<Keyword MajorTopicYN="N">Secondary cell wall</Keyword>
<Keyword MajorTopicYN="N">Sugar composition</Keyword>
<Keyword MajorTopicYN="N">Uronic acid</Keyword>
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